2F5E
Bacillus subtilis manganese transport regulator (MNTR) bound to manganese, AB conformation, pH 6.5
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | RIGAKU RU300 |
Temperature [K] | 100 |
Detector technology | IMAGE PLATE |
Collection date | 2003-06-17 |
Detector | RIGAKU RAXIS IV |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 49.400, 46.010, 74.360 |
Unit cell angles | 90.00, 92.93, 90.00 |
Refinement procedure
Resolution | 19.960 - 2.200 |
R-factor | 0.237 |
Rwork | 0.237 |
R-free | 0.28600 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1on1 |
RMSD bond length | 0.006 |
RMSD bond angle | 1.000 |
Data reduction software | MOSFLM |
Data scaling software | SCALA |
Phasing software | CNS |
Refinement software | CNS |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 20.000 | 2.340 |
High resolution limit [Å] | 2.200 | 2.200 |
Rmerge | 0.083 | 0.325 |
Number of reflections | 16728 | |
<I/σ(I)> | 7.7 | 2.2 |
Completeness [%] | 97.3 | 97.1 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 295 | 15% PEG 4000, 0.1 M MAGNESIUM CHLORIDE, 0.1 M CACODYLATE, PH 6.50, VAPOR DIFFUSION, HANGING DROP, TEMPERATURE 295K |