2EHG

Crystal structure of hyperthermophilic archaeal RNase HI

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SPRING-8 BEAMLINE BL41XU
Synchrotron site	SPring-8
Beamline	BL41XU
Temperature [K]	100
Detector technology	CCD
Collection date	2005-10-03
Detector	ADSC QUANTUM 315
Wavelength(s)	1.0
Spacegroup name	P 43
Unit cell lengths	39.206, 39.206, 91.147
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	26.520 - 1.600
R-factor	0.194
Rwork	0.194
R-free	0.21800
Structure solution method	MAD
RMSD bond length	0.005
RMSD bond angle	1.000
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	SOLVE
Refinement software	CNS (1.0)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	26.520	1.570
High resolution limit [Å]	1.520	1.520
Rmerge	0.047	0.199
Number of reflections	21009
<I/σ(I)>	28.1	3.8
Completeness [%]	99.1	93.9

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	10.5	277	0.1M CAPS-NaOH, 30% PEG400, pH 10.5, VAPOR DIFFUSION, SITTING DROP, temperature 277K
1	VAPOR DIFFUSION, SITTING DROP	10.5	277	0.1M CAPS-NaOH, 30% PEG400, pH 10.5, VAPOR DIFFUSION, SITTING DROP, temperature 277K
1	VAPOR DIFFUSION, SITTING DROP	10.5	277	0.1M CAPS-NaOH, 30% PEG400, pH 10.5, VAPOR DIFFUSION, SITTING DROP, temperature 277K