2E52
Crystal structural analysis of HindIII restriction endonuclease in complex with cognate DNA at 2.0 angstrom resolution
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | PHOTON FACTORY BEAMLINE AR-NW12A |
Synchrotron site | Photon Factory |
Beamline | AR-NW12A |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2006-06-16 |
Detector | MAR CCD 165 mm |
Wavelength(s) | 1.0 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 83.125, 131.532, 93.718 |
Unit cell angles | 90.00, 111.20, 90.00 |
Refinement procedure
Resolution | 39.684 - 2.000 |
R-factor | 0.17672 |
Rwork | 0.175 |
R-free | 0.21665 |
Structure solution method | SAD |
RMSD bond length | 0.015 |
RMSD bond angle | 1.503 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | SOLVE |
Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 39.684 | 2.070 |
High resolution limit [Å] | 2.000 | 2.000 |
Rmerge | 0.055 | 0.156 |
Number of reflections | 126130 | |
<I/σ(I)> | 29.2 | 11.8 |
Completeness [%] | 100.0 | 100 |
Redundancy | 7.7 | 7.5 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 293 | 18% PEG 3350, 0.08M Ammonium Acetate, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
Crystallization Reagents
ID | crystal ID | solution ID | reagent name | concentration | details |
1 | 1 | 1 | PEG 3350 | ||
2 | 1 | 1 | Ammonium Acetate | ||
3 | 1 | 1 | HOH | ||
4 | 1 | 2 | PEG 3350 | ||
5 | 1 | 2 | Ammonium Acetate | ||
6 | 1 | 2 | HOH |