2E1W

Crystal structure of adenosine deaminase complexed with potent inhibitors

Replaces:  1V78

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SPRING-8 BEAMLINE BL24XU
Synchrotron site	SPring-8
Beamline	BL24XU
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	2003-10-25
Detector	RIGAKU RAXIS V
Wavelength(s)	0.8266
Spacegroup name	P 43 21 2
Unit cell lengths	78.410, 78.410, 137.460
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	29.790 - 2.500
R-factor	0.22
Rwork	0.218
R-free	0.24600
Structure solution method	MOLECULAR REPLACEMENT
Data reduction software	CrystalClear ((MSC/RIGAKU))
Data scaling software	CrystalClear ((MSC/RIGAKU))
Phasing software	AMoRE
Refinement software	CNX

Data quality characteristics

	Overall
Low resolution limit [Å]	29.790
High resolution limit [Å]	2.500
Number of reflections	11679
Completeness [%]	75.4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	6	293	ammonium sulphate, PEG400, MES buffer, pH 6.0, VAPOR DIFFUSION, SITTING DROP, temperature 293K