2DXI
2.2 A crystal structure of glutamyl-tRNA synthetase from Thermus thermophilus complexed with tRNA(Glu), ATP, and L-glutamol
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SPRING-8 BEAMLINE BL41XU |
Synchrotron site | SPring-8 |
Beamline | BL41XU |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2004-02-10 |
Detector | MARRESEARCH |
Wavelength(s) | 1 |
Spacegroup name | C 2 2 21 |
Unit cell lengths | 110.615, 219.119, 135.816 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 19.970 - 2.200 |
R-factor | 0.214 |
Rwork | 0.214 |
R-free | 0.25700 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1n78 |
RMSD bond length | 0.010 |
RMSD bond angle | 1.400 |
Data reduction software | HKL-2000 |
Data scaling software | SCALEPACK |
Phasing software | CNS |
Refinement software | CNS |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.180 |
High resolution limit [Å] | 2.100 | 2.100 |
Rmerge | 0.085 | |
Number of reflections | 94855 | |
<I/σ(I)> | 19.2 | 1.89 |
Completeness [%] | 99.6 | 100 |
Redundancy | 4.3 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6.7 | 293 | PEG1500, MOPS-NA, AMMONIUM SULFATE, MPD, pH 6.70, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
Crystallization Reagents
ID | crystal ID | solution ID | reagent name | concentration | details |
1 | 1 | 1 | PEG1500 | ||
2 | 1 | 1 | Mops-Na | ||
3 | 1 | 1 | ammonium sulfate | ||
4 | 1 | 1 | MPD | ||
5 | 1 | 1 | HOH | ||
6 | 1 | 2 | PEG1500 | ||
7 | 1 | 2 | ammonium sulfate | ||
8 | 1 | 2 | MPD | ||
9 | 1 | 2 | HOH |