2DQU
Crystal form II: high resolution crystal structure of the complex of the hydrolytic antibody Fab 6D9 and a transition-state analog
Replaces: 1HYYExperimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | PHOTON FACTORY BEAMLINE BL-6A |
Synchrotron site | Photon Factory |
Beamline | BL-6A |
Temperature [K] | 293 |
Detector technology | IMAGE PLATE |
Collection date | 1994-10-15 |
Detector | FUJI |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 58.700, 63.040, 56.560 |
Unit cell angles | 90.00, 98.75, 90.00 |
Refinement procedure
Resolution | 40.000 - 1.700 |
R-factor | 0.207 |
Rwork | 0.207 |
R-free | 0.24600 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2dqt |
RMSD bond length | 0.008 |
RMSD bond angle | 1.500 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | X-PLOR |
Refinement software | CNS (1.1) |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 40.000 |
High resolution limit [Å] | 1.700 |
Rmerge | 0.082 |
Number of reflections | 38032 |
Completeness [%] | 89.0 |
Redundancy | 7.3 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | 8.3 | THE PRE-FORMED COMPLEX WAS CRYSTALLIZED FROM 17% PEG 4000, 50MM TRIS, 0.1MM EDTA, PH 8.3 |