2DOQ
crystal structure of Sfi1p/Cdc31p complex
Experimental procedure
Experimental method | MAD |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE ID14-4 |
Synchrotron site | ESRF |
Beamline | ID14-4 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2005-11-21 |
Detector | ADSC |
Wavelength(s) | 0.9794, 0.9393 |
Spacegroup name | C 2 2 21 |
Unit cell lengths | 87.283, 92.961, 189.358 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 94.490 - 3.000 |
R-factor | 0.26057 |
Rwork | 0.259 |
R-free | 0.29899 |
Structure solution method | MAD |
RMSD bond length | 0.010 |
RMSD bond angle | 1.253 |
Data reduction software | MOSFLM |
Data scaling software | CCP4 ((SCALA)) |
Phasing software | SnB |
Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 94.490 | 3.430 |
High resolution limit [Å] | 3.250 | 3.250 |
Rmerge | 0.110 | 0.480 |
Number of reflections | 12505 | |
<I/σ(I)> | 3.8 | 1.3 |
Completeness [%] | 99.7 | 99.7 |
Redundancy | 6.8 | 6.8 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 6.2 | 277 | 9% iso-propanol, 0.1M MES pH=6.2, 0.2M Ca(OAc)2, VAPOR DIFFUSION, SITTING DROP, temperature 277K |