2DH6
Crystal structure of E. coli Apo-TrpB
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SPRING-8 BEAMLINE BL44XU |
Synchrotron site | SPring-8 |
Beamline | BL44XU |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2003-10-26 |
Detector | Bruker DIP-6040 |
Wavelength(s) | 0.9 |
Spacegroup name | P 43 2 2 |
Unit cell lengths | 110.886, 110.886, 102.213 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 42.820 - 3.000 |
R-factor | 0.206 |
Rwork | 0.206 |
R-free | 0.25400 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | CHAIN B of 1BKS |
RMSD bond length | 0.012 |
RMSD bond angle | 1.700 |
Data reduction software | MOSFLM |
Data scaling software | CCP4 ((SCALA)) |
Phasing software | CNS |
Refinement software | CNS (1.1) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 46.410 | 3.160 |
High resolution limit [Å] | 3.000 | 3.000 |
Rmerge | 0.074 | 0.389 |
Number of reflections | 13266 | |
Completeness [%] | 99.9 | 100 |
Redundancy | 5.2 | 5.3 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 288 | lithium sulfate, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 288K |