2CL8
Dectin-1 in complex with beta-glucan
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU MICROMAX-007 |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2005-12-13 |
| Detector | MARRESEARCH |
| Spacegroup name | P 32 2 1 |
| Unit cell lengths | 83.998, 83.998, 117.404 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 72.740 - 2.800 |
| R-factor | 0.194 |
| Rwork | 0.191 |
| R-free | 0.24500 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2bpe |
| RMSD bond length | 0.020 |
| RMSD bond angle | 1.836 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.900 |
| High resolution limit [Å] | 2.800 | 2.800 |
| Rmerge | 0.240 | 0.920 |
| Number of reflections | 12244 | |
| <I/σ(I)> | 18.4 | 3.5 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 31.4 | 30.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 4 | 2M SODIUM CHLORIDE, 0.1M CITRIC ACID PH4.0, pH 4.00 |
