2CHZ

A pharmacological map of the PI3-K family defines a role for p110alpha in signaling: The structure of complex of phosphoinositide 3-kinase gamma with inhibitor PIK-93

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID14-4
Synchrotron site	ESRF
Beamline	ID14-4
Temperature [K]	100
Detector technology	CCD
Collection date	2005-01-05
Detector	ADSC CCD
Spacegroup name	C 1 2 1
Unit cell lengths	144.025, 68.114, 106.351
Unit cell angles	90.00, 95.21, 90.00

Refinement procedure

Resolution	71.800 - 2.600
R-factor	0.246
Rwork	0.244
R-free	0.30200
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1e8z
RMSD bond length	0.012
RMSD bond angle	1.212
Data reduction software	MOSFLM
Data scaling software	SCALA
Phasing software	AMoRE
Refinement software	REFMAC (5.2.0005)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	71.800	2.740
High resolution limit [Å]	2.600	2.600
Rmerge	0.070	0.360
Number of reflections	31614
<I/σ(I)>	14.3	2.7
Completeness [%]	99.8	99.8
Redundancy	3.7	3.7

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION	7.5		VAPOUR DIFFUSION 1 MICROLITER PROTEIN PLUS 1 MICROLITER RESERVOIR RESERVOIR: 17% PEG 4000, 250 MM AMMONIUM SULFATE AND 100 MM TRIS PH 7.5 PROTEIN: 4 MG/ML IN 0.5 MM AMMONIUM SULFATE, 20 MM TRIS PH 7.2, 1% ETHYLENE GLYCOL, 0.02% CHAPS AND 5 MM DTT