2CD0

STRUCTURE OF HUMAN LAMBDA-6 LIGHT CHAIN DIMER WIL

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 19-ID
Synchrotron site	APS
Beamline	19-ID
Temperature [K]	100
Detector technology	CCD
Collection date	1998-09
Detector	CUSTOM-MADE
Spacegroup name	H 3 2
Unit cell lengths	147.900, 147.900, 46.600
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	8.000 - 1.800
Rwork	0.289
R-free	0.35100
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	MCG (1DCL): USED ONLY VL DIMER WITH CDR LOOPS DELETED.
RMSD bond length	0.007
RMSD bond angle	25.500 *
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	AMoRE
Refinement software	CNS (0.3)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	20.000	1.760
High resolution limit [Å]	1.700	1.700
Rmerge	0.040	0.232
Number of reflections	21478
<I/σ(I)>	48	19
Completeness [%]	97.6	96.4
Redundancy	21.8	9

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion *	5.6		1.8M AMMONIUM SULPHATE, 0.1 M SODIUM CITRATE PH 5.6, 0.01 M CADMIUM CHLORIDE

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	reservoir	ammonium sulfate
2	1	reservoir	PEG6000