2C59
gdp-mannose-3', 5' -epimerase (arabidopsis thaliana), with gdp-alpha-d-mannose and gdp-beta-l-galactose bound in the active site.
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | RIGAKU MICROMAX-007 |
Temperature [K] | 100 |
Detector technology | IMAGE PLATE |
Collection date | 2004-11-15 |
Detector | RIGAKU IMAGE PLATE |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 62.808, 83.812, 66.293 |
Unit cell angles | 90.00, 98.91, 90.00 |
Refinement procedure
Resolution | 29.880 - 2.000 |
R-factor | 0.151 |
Rwork | 0.147 |
R-free | 0.22300 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2c54 |
RMSD bond length | 0.020 |
RMSD bond angle | 1.838 |
Data reduction software | MOSFLM |
Data scaling software | SCALA |
Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 30.500 | 2.110 |
High resolution limit [Å] | 2.000 | 2.000 |
Rmerge | 0.080 | 0.450 |
Number of reflections | 45789 | |
<I/σ(I)> | 7.9 | 1.7 |
Completeness [%] | 99.6 | 97.7 |
Redundancy | 3.6 | 3.5 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 6 | 100 MM BIS-TRIS PH 6.0, 2.2 M AMMONIUM SULPHATE, SITTING DROP VAPOUR DIFFUSION. CRYOPROTECTED WITH 6M SODIUM FORMATE. |