2C2A
Structure of the entire cytoplasmic portion of a sensor histidine kinase protein
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | NSLS BEAMLINE X4A |
Synchrotron site | NSLS |
Beamline | X4A |
Temperature [K] | 100 |
Detector technology | CCD |
Detector | ADSC CCD |
Spacegroup name | C 2 2 21 |
Unit cell lengths | 79.292, 162.082, 42.525 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 20.000 - 1.900 |
R-factor | 0.2465 |
Rwork | 0.246 |
R-free | 0.27520 |
Structure solution method | MAD |
RMSD bond length | 0.011 |
RMSD bond angle | 1.638 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | SOLVE |
Refinement software | CNS (1.0) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 20.000 | 2.000 |
High resolution limit [Å] | 1.900 | 1.900 |
Rmerge | 0.060 | 0.270 |
Number of reflections | 21892 | |
<I/σ(I)> | 13.8 | 2.7 |
Completeness [%] | 98.0 | 99.7 |
Redundancy | 13.4 | 5.2 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | 6.5 | 1.25 M LI2SO4, 100 MM AMMONIUM ACETATE AT PH 6.5 |