2BL1
Crystal structure of a putative phosphinothricin Acetyltransferase (PA4866) from Pseudomonas aeruginosa PAC1
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SRS BEAMLINE PX14.2 |
Synchrotron site | SRS |
Beamline | PX14.2 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2004-03-23 |
Detector | ADSC CCD |
Spacegroup name | P 43 21 2 |
Unit cell lengths | 79.990, 79.990, 61.730 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 6.000 - 2.000 |
R-factor | 0.1974 |
Rwork | 0.197 |
R-free | 0.22940 |
Structure solution method | SAD |
Starting model (for MR) | NONE |
RMSD bond length | 0.019 |
RMSD bond angle | 1.410 |
Data reduction software | DENZO |
Data scaling software | SCALA |
Phasing software | SOLVE |
Refinement software | CNS (1.1) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 70.000 | 2.050 |
High resolution limit [Å] | 2.000 | 2.000 |
Rmerge | 0.100 | 0.280 |
Number of reflections | 13388 | |
<I/σ(I)> | 4.6 | 2.6 |
Completeness [%] | 96.1 | 96.1 |
Redundancy | 12.8 | 12.7 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7.3 | PROTEIN WAS CRYSTALLISED USING HANGING DROP VAPOUR DIFFUSION. RESERVOIR SOLUTION CONTAINED 1ML OF 0.1M HEPES AT PH7.3, 23-27% PEG 8000 AND 0.1% AZIDE. DROP SIZE WAS 1 MICROLITRE, TO WHICH 1 MICROLITRE OF PROTEIN SOLUTION AT 10 MG/ML WAS ADDED. |