2B0L
C-terminal DNA binding domain of transcriptional pleiotropic repressor CodY.
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | RIGAKU RU200 |
Temperature [K] | 298 |
Detector technology | IMAGE PLATE |
Collection date | 2005-08-12 |
Detector | MARRESEARCH |
Wavelength(s) | 1.54178 |
Spacegroup name | P 4 2 2 |
Unit cell lengths | 68.128, 68.128, 164.903 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 10.000 - 2.900 |
R-factor | 0.20445 |
Rwork | 0.202 |
R-free | 0.25033 |
Structure solution method | MAD |
RMSD bond length | 0.011 |
RMSD bond angle | 1.544 |
Data reduction software | HKL-2000 |
Data scaling software | SCALEPACK |
Phasing software | HKL2Map |
Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 25.000 | 2.974 |
High resolution limit [Å] | 2.900 | 2.900 |
Rmerge | 0.072 | 0.655 |
Number of reflections | 8705 | |
<I/σ(I)> | 15.4 | 1.2 |
Completeness [%] | 94.4 | 84.3 |
Redundancy | 3.7 | 2.4 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 291 | ammoium sulphate, sodium citrate, NaCl, glycerol, tacsimate, TRIS, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 291K |
1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 291 | ammoium sulphate, sodium citrate, NaCl, glycerol, tacsimate, TRIS, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 291K |
1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 291 | ammoium sulphate, sodium citrate, NaCl, glycerol, tacsimate, TRIS, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 291K |