2ARM
Crystal Structure of the Complex of Phospholipase A2 with a natural compound atropine at 1.2 A resolution
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | EMBL/DESY, HAMBURG BEAMLINE X11 |
Synchrotron site | EMBL/DESY, Hamburg |
Beamline | X11 |
Temperature [K] | 200 |
Detector technology | CCD |
Collection date | 2004-04-20 |
Detector | MARRESEARCH |
Wavelength(s) | 0.80 |
Spacegroup name | P 43 |
Unit cell lengths | 52.350, 52.350, 47.828 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 51.990 - 1.230 |
R-factor | 0.18909 |
Rwork | 0.189 |
R-free | 0.20444 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.008 |
RMSD bond angle | 1.946 |
Data reduction software | HKL-2000 |
Data scaling software | SCALEPACK |
Phasing software | AMoRE |
Refinement software | REFMAC (5.0) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 51.990 | 1.260 |
High resolution limit [Å] | 1.230 | 1.230 |
Number of reflections | 36667 | |
Completeness [%] | 100.0 | 100 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 7 | 298 | ammonium sulphate and PEG 4000, pH 7.0, VAPOR DIFFUSION, SITTING DROP, temperature 298K |