2AR7
Crystal structure of human adenylate kinase 4, AK4
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SLS BEAMLINE X10SA |
Synchrotron site | SLS |
Beamline | X10SA |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2005-04-23 |
Detector | MARMOSAIC 225 mm CCD |
Wavelength(s) | 0.968 |
Spacegroup name | C 2 2 21 |
Unit cell lengths | 89.738, 95.514, 140.873 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 47.950 - 2.150 |
R-factor | 0.16934 |
Rwork | 0.166 |
R-free | 0.22995 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2bbw |
RMSD bond length | 0.014 |
RMSD bond angle | 1.455 |
Data reduction software | HKL-2000 |
Data scaling software | SCALEPACK |
Phasing software | PHASER |
Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 47.950 | 2.230 |
High resolution limit [Å] | 2.150 | 2.150 |
Number of reflections | 32401 | |
<I/σ(I)> | 19.5 | 2.1 |
Completeness [%] | 97.2 | 82.1 |
Redundancy | 6.1 | 3.8 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 7 | 277 | sodium succinate, pH 7, VAPOR DIFFUSION, SITTING DROP, temperature 277K |