2AJR
Crystal structure of Possible 1-phosphofructokinase (EC 2.7.1.56) (tm0828) from Thermotoga Maritima at 2.46 A resolution
Replaces: 1O14Experimental procedure
Experimental method | MAD |
Source type | SYNCHROTRON |
Source details | SSRL BEAMLINE BL11-1 |
Synchrotron site | SSRL |
Beamline | BL11-1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2002-06-17 |
Detector | ADSC QUANTUM 315 |
Wavelength(s) | 0.885567,0.976224 |
Spacegroup name | P 41 21 2 |
Unit cell lengths | 115.796, 115.796, 124.828 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 28.490 - 2.460 |
Rwork | 0.193 |
R-free | 0.24000 |
Structure solution method | MAD |
RMSD bond length | 0.012 |
RMSD bond angle | 1.274 |
Data reduction software | MOSFLM |
Data scaling software | SCALA |
Phasing software | SnB |
Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 29.200 | 2.520 |
High resolution limit [Å] | 2.400 | 2.460 |
Rmerge | 0.109 | 0.633 |
Number of reflections | 31322 | |
<I/σ(I)> | 5.9 | 1.2 |
Completeness [%] | 99.5 | 95 |
Redundancy | 6.3 | 3.5 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | 6.5 | 293 | 16% PEG-8000, 0.08M sodium cacodylate, 0.16M magnesium acetate tetrahydrate, 20% glyerol anhyrous , VAPOR DIFFUSION,SITTING DROP,NANODROP, temperature 293K, pH 6.5 |