2ADV
Crystal Structures Of Glutaryl 7-Aminocephalosporanic Acid Acylase: mutational study of activation mechanism
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | PAL/PLS BEAMLINE 4A |
Synchrotron site | PAL/PLS |
Beamline | 4A |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2005-05-23 |
Detector | ADSC QUANTUM 210 |
Wavelength(s) | 0.95 |
Spacegroup name | P 41 21 2 |
Unit cell lengths | 73.775, 73.775, 384.646 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 48.220 - 2.244 |
R-factor | 0.1848 |
Rwork | 0.183 |
R-free | 0.21035 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.012 |
RMSD bond angle | 1.264 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | CNS |
Refinement software | CNS |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.290 |
High resolution limit [Å] | 2.240 | 2.240 |
Rmerge | 0.080 | |
Number of reflections | 49679 | |
Completeness [%] | 94.5 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 295 | PEG 3350, Tris, magnesium chloride, cacodylate, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 295K |