2AAD

THE ROLE OF HISTIDINE-40 IN RIBONUCLEASE T1 CATALYSIS: THREE-DIMENSIONAL STRUCTURES OF THE PARTIALLY ACTIVE HIS40LYS MUTANT

Experimental procedure

Spacegroup name	P 1 21 1
Unit cell lengths	49.200, 48.190, 40.160
Unit cell angles	90.00, 90.26, 90.00

Refinement procedure

Resolution	10.000 - 2.000
R-factor	0.16
RMSD bond length	0.023
RMSD bond angle	0.056 *
Refinement software	PROLSQ

Data quality characteristics

	Overall	Outer shell
High resolution limit [Å]	2.000 *	2.000 *
Rmerge	0.093 *
Completeness [%]		77 *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	10 (mg/mL)
2	1	drop	sodium acetate	20 (mM)
3	1	drop	calcium acetate	2 (mM)
4	1	drop	2'-GMP	0.2 (%(w/v))
5	1	reservoir	MPD	53 (%(v/v))