2A6L
Dihydrodipicolinate synthase (E. coli)- mutant R138H
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | RIGAKU |
Detector technology | IMAGE PLATE |
Detector | RIGAKU RAXIS IV |
Wavelength(s) | 1.5418 |
Spacegroup name | P 31 2 1 |
Unit cell lengths | 121.016, 121.016, 111.414 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 32.270 - 2.050 |
R-factor | 0.184 |
Rwork | 0.183 |
R-free | 0.21280 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.007 |
RMSD bond angle | 1.106 |
Phasing software | AMoRE |
Refinement software | REFMAC (5.2.0003) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 33.300 | 33.300 |
High resolution limit [Å] | 2.050 | 2.050 |
Rmerge | 0.087 | |
Number of reflections | 59240 | |
<I/σ(I)> | 10.4 | |
Completeness [%] | 99.7 | 99.7 |
Redundancy | 4.2 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION | 10 | 285 | protein solution (~5 mg/ml in Tris.HCl 20 mM, pH 8, 2.5 uL), precipitant (K2HPO4 1.8 M, pH 10, 1.2 uL), and N-octyl- -R-glucopyranoside (6% w/v, 0.6 uL), VAPOR DIFFUSION, temperature 12K |