233D
THE CRYSTAL STRUCTURE ANALYSIS OF D(CGCGAASSCGCG)2: A SYNTHETIC DNA DODECAMER DUPLEX CONTAINING FOUR 4'-THIO-2'-DEOXYTHYMIDINE NUCLEOTIDES
Experimental procedure
Source type | ROTATING ANODE |
Source details | RIGAKU RU200 |
Detector technology | IMAGE PLATE |
Detector | RIGAKU RAXIS II |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 24.993, 40.364, 65.993 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 8.000 - 2.400 |
R-factor | 0.201 |
Rwork | 0.201 |
RMSD bond length | 0.018 |
RMSD bond angle | 3.530 |
Refinement software | X-PLOR |
Data quality characteristics
Overall | |
High resolution limit [Å] | 2.400 |
Rmerge | 0.065 * |
Number of reflections | 2820 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 6 | pH 6.00, VAPOR DIFFUSION, SITTING DROP |
Crystallization Reagents
ID | crystal ID | solution ID | reagent name | concentration | details |
1 | 1 | 1 | WATER | ||
2 | 1 | 1 | MPD | ||
3 | 1 | 1 | NA CACODYLATE | ||
4 | 1 | 1 | MGCL2 | ||
5 | 1 | 1 | SPERMINE_HCL | ||
6 | 1 | 2 | WATER | ||
7 | 1 | 2 | MPD |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | drop | dodecanucleotide | 0.5 (mM) | |
2 | 1 | drop | sodium cacodylate | 14 (mM) | |
3 | 1 | drop | magnesium chloride | 17 (mM) | |
4 | 1 | drop | spermine tetrahydrochloride | 1 (mM) | |
5 | 1 | drop | MPD | 17 (%(v/v)) | |
6 | 1 | reservoir | MPD | 50 (%) |