1ZX6
High-resolution crystal structure of yeast Pin3 SH3 domain
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | EMBL/DESY, HAMBURG BEAMLINE X11 |
Synchrotron site | EMBL/DESY, HAMBURG |
Beamline | X11 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2005-04-20 |
Detector | MARRESEARCH |
Wavelength(s) | 0.813 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 89.040, 27.360, 24.000 |
Unit cell angles | 90.00, 102.87, 90.00 |
Refinement procedure
Resolution | 20.000 - 1.600 |
R-factor | 0.18347 |
Rwork | 0.182 |
R-free | 0.22089 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | : 1oot |
RMSD bond length | 0.013 |
RMSD bond angle | 1.451 |
Data reduction software | XDS |
Data scaling software | XDS |
Phasing software | MOLREP |
Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 20.000 | 1.700 |
High resolution limit [Å] | 1.600 | 1.600 |
Number of reflections | 7155 | |
<I/σ(I)> | 7.9 | 2.6 |
Completeness [%] | 94.2 | 96.8 |
Redundancy | 2 | 1.9 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 295 | VAPOR DIFFUSION, HANGING DROP, temperature 295K |