1ZUY
High-resolution structure of yeast Myo5 SH3 domain
Experimental procedure
Experimental method | MAD |
Source type | SYNCHROTRON |
Source details | EMBL/DESY, HAMBURG BEAMLINE X11 |
Synchrotron site | EMBL/DESY, HAMBURG |
Beamline | X11 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2004-02-03 |
Detector | MARRESEARCH |
Wavelength(s) | 0.813 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 27.060, 42.250, 90.910 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 20.000 - 1.390 |
R-factor | 0.16829 |
Rwork | 0.166 |
R-free | 0.20581 |
Structure solution method | MAD |
RMSD bond length | 0.017 |
RMSD bond angle | 1.752 |
Data scaling software | XDS |
Phasing software | SOLVE |
Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 20.000 | 1.430 |
High resolution limit [Å] | 1.390 | 1.390 |
Number of reflections | 21535 | |
<I/σ(I)> | 20 | 3.3 |
Completeness [%] | 99.0 | 90.6 |
Redundancy | 6.1 | 3.8 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 295 | ammonium sulfate, isopropanol, VAPOR DIFFUSION, HANGING DROP, temperature 295K |