1ZUQ
Contribution to Structure and Catalysis of Tyrosine 34 in Human Manganese Superoxide Dismutase
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRL BEAMLINE BL9-1 |
Synchrotron site | SSRL |
Beamline | BL9-1 |
Temperature [K] | 100 |
Spacegroup name | P 61 2 2 |
Unit cell lengths | 79.626, 79.626, 241.612 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 39.570 - 2.000 |
R-factor | 0.235 |
Rwork | 0.235 |
R-free | 0.27400 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1noj |
RMSD bond length | 0.005 |
RMSD bond angle | 1.200 |
Data reduction software | HKL-2000 |
Data scaling software | SCALEPACK |
Phasing software | AMoRE |
Refinement software | CNS |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 39.570 | 2.130 |
High resolution limit [Å] | 2.000 | 2.000 |
Rmerge | 0.055 | 0.226 |
Number of reflections | 30942 | |
<I/σ(I)> | 28.6 | 5.4 |
Completeness [%] | 97.6 | 96.8 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | 7.5 | 293 | 2.5 M ammonium sulfate, 100mM imidazole/malate buffer, pH 7.5, VAPOR DIFFUSION, temperature 293K, pH 7.50 |