1ZQ7
X-Ray Crystal Structure of Protein Q8PZK8 from Methanosarcina mazei. Northeast Structural Genomics Consortium Target MaR9.
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X4A |
| Synchrotron site | NSLS |
| Beamline | X4A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2005-05-06 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 0.97902, 0.97924, 0.96788 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 137.880, 57.576, 115.654 |
| Unit cell angles | 90.00, 110.60, 90.00 |
Refinement procedure
| Resolution | 29.830 - 2.110 |
| R-factor | 0.208 |
| Rwork | 0.208 |
| R-free | 0.24800 |
| Structure solution method | MAD |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.400 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | SOLVE |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 2.180 |
| High resolution limit [Å] | 2.100 | 2.100 |
| Rmerge | 0.055 | |
| Number of reflections | 76303 | |
| <I/σ(I)> | 13.2 | |
| Completeness [%] | 89.6 | 78.5 |
| Redundancy | 2.53 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 277 | 200 mM ammonium sulfate, 18% PEG 3350, 50 Nm Hepes, 3% 1,3 Butanedio, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 277K |
