1ZNC
HUMAN CARBONIC ANHYDRASE IV
Experimental procedure
Source type | ROTATING ANODE |
Source details | RIGAKU RUH2R |
Temperature [K] | 298 |
Detector technology | IMAGE PLATE |
Detector | RIGAKU |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 89.500, 47.700, 141.000 |
Unit cell angles | 90.00, 106.30, 90.00 |
Refinement procedure
Resolution | 20.000 - 2.800 |
R-factor | 0.197 |
Rwork | 0.197 |
R-free | 0.25200 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | POLYALANINE MODEL OF CAII MISSING N-TERMINAL 30 |
RMSD bond length | 0.007 |
RMSD bond angle | 24.800 * |
Data reduction software | MOSFLM |
Data scaling software | CCP4 ((AGROVATA) |
Phasing software | X-PLOR (3.1) |
Refinement software | X-PLOR (3.1) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 20.000 | 2.870 |
High resolution limit [Å] | 2.800 | 2.800 |
Rmerge | 0.080 | 0.253 |
Total number of observations | 46250 * | |
Number of reflections | 13626 | |
Completeness [%] | 95.0 | 91.8 |
Redundancy | 3.4 | 3.3 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | Vapor diffusion, hanging drop * | 5.1 | 25% PEG 3,350; 100MM NA ACETATE PH 5.1 |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | drop | protein | 5-8 (mg/ml) | |
2 | 1 | drop | Tris sulfate | 50 (mM) | |
3 | 1 | reservoir | PEG3350 | 25 (%) | |
4 | 1 | reservoir | sodium acetate | 100 (mM) | |
5 | 1 | reservoir | benzamidine | 2 (mM) | |
6 | 1 | reservoir | n-octyl beta-D-glucopyranoside | (mM) |