1ZLB
Crystal structure of catalytically-active phospholipase A2 in the absence of calcium
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | EMBL/DESY, HAMBURG BEAMLINE X11 |
Synchrotron site | EMBL/DESY, Hamburg |
Beamline | X11 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2004-08-14 |
Detector | MARRESEARCH |
Wavelength(s) | 0.80 |
Spacegroup name | C 2 2 21 |
Unit cell lengths | 39.964, 53.794, 89.816 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 10.000 - 0.970 |
R-factor | 0.168 |
Rwork | 0.151 |
R-free | 0.19610 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.017 |
RMSD bond angle | 0.040 |
Data scaling software | SCALEPACK |
Phasing software | AMoRE |
Refinement software | SHELXL-97 |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 10.000 | 0.990 |
High resolution limit [Å] | 0.970 | 0.970 |
Rmerge | 0.068 | 0.477 |
Number of reflections | 57413 | |
Completeness [%] | 99.3 | 97.8 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 8 | 293 | PEG 3350, NaCl, EDTA, tris, pH 8.0, VAPOR DIFFUSION, HANGING DROP, temperature 293K |