1Z9Z
Crystal structure of yeast sla1 SH3 domain 3
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | EMBL/DESY, HAMBURG BEAMLINE BW7A |
Synchrotron site | EMBL/DESY, HAMBURG |
Beamline | BW7A |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2004-11-23 |
Detector | MARRESEARCH |
Wavelength(s) | 0.9781 |
Spacegroup name | P 41 21 2 |
Unit cell lengths | 83.990, 83.990, 50.140 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 15.000 - 1.950 |
R-factor | 0.18036 |
Rwork | 0.178 |
R-free | 0.22902 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.012 |
RMSD bond angle | 1.350 |
Data scaling software | XDS |
Phasing software | AMoRE |
Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 20.000 | 10.000 |
High resolution limit [Å] | 1.900 | 1.900 |
Rmerge | 0.066 | |
Number of reflections | 12025 | |
<I/σ(I)> | 23.44 | |
Completeness [%] | 99.6 | 99.6 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 5 | 295 | ammonium sulphate, sodium citrate, potassium/sodium tartrate, pH 5.0, VAPOR DIFFUSION, HANGING DROP, temperature 295.0K |