1Z8F
Guanylate Kinase Double Mutant A58C, T157C from Mycobacterium tuberculosis (Rv1389)
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ALS BEAMLINE 8.2.2 |
Synchrotron site | ALS |
Beamline | 8.2.2 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2004-11-21 |
Detector | ADSC QUANTUM 315 |
Wavelength(s) | 0.9537 |
Spacegroup name | I 2 3 |
Unit cell lengths | 112.306, 112.306, 112.306 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 79.310 - 2.500 |
R-factor | 0.18314 |
Rwork | 0.181 |
R-free | 0.23576 |
Structure solution method | Difference Fourier Map |
Starting model (for MR) | 1s4q |
RMSD bond length | 0.014 |
RMSD bond angle | 1.444 |
Data scaling software | SCALEPACK |
Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 90.000 | 2.590 |
High resolution limit [Å] | 2.500 | 2.500 |
Number of reflections | 8301 | |
<I/σ(I)> | 35.6 | 5.8 |
Completeness [%] | 100.0 | 100 |
Redundancy | 10.9 | 10.3 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 8 | 298 | 2.6M sodium formate, 2% glycerol, pH 8.0, VAPOR DIFFUSION, SITTING DROP, temperature 298.0K |