1Z3T
Structure of Phanerochaete chrysosporium cellobiohydrolase Cel7D (CBH58) in complex with cellobiose
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE ID14-4 |
Synchrotron site | ESRF |
Beamline | ID14-4 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 1999-02-07 |
Detector | ADSC QUANTUM 4 |
Wavelength(s) | 0.9370 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 87.414, 46.842, 99.488 |
Unit cell angles | 90.00, 102.99, 90.00 |
Refinement procedure
Resolution | 40.000 - 1.700 |
R-factor | 0.16125 |
Rwork | 0.159 |
R-free | 0.19866 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1gpi |
RMSD bond length | 0.026 |
RMSD bond angle | 2.062 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | CNS |
Refinement software | REFMAC (5.0) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 1.730 |
High resolution limit [Å] | 1.700 | 1.700 |
Rmerge | 0.098 | 0.321 |
Number of reflections | 43328 | |
<I/σ(I)> | 11 | 5.8 |
Completeness [%] | 99.6 | 93.1 |
Redundancy | 4.3 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7 | 294 | TRIS-HCl, calcium chloride, PEG 5000, glycerol, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 294K |