1YYW
Crystal structure of RNase III from Aquifex aeolicus complexed with double stranded RNA at 2.8-Angstrom Resolution
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 22-ID |
Synchrotron site | APS |
Beamline | 22-ID |
Detector technology | CCD |
Collection date | 2003-12-14 |
Detector | BRUKER PROTEUM 300 |
Wavelength(s) | 1.000 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 86.428, 95.906, 166.004 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 29.980 - 2.800 |
R-factor | 0.246 |
Rwork | 0.243 |
R-free | 0.31400 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | PDB 1I4S |
RMSD bond length | 0.005 |
RMSD bond angle | 1.000 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | AMoRE |
Refinement software | CNS (1.0) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 29.980 | 2.900 |
High resolution limit [Å] | 2.800 | 2.800 |
Rmerge | 0.084 | 0.361 |
Number of reflections | 31417 | |
<I/σ(I)> | 12.6 | 1.2 |
Completeness [%] | 90.5 | 44.8 |
Redundancy | 5.2 | 1.7 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 5.6 | 292 | PEG4000, iso-propanol, tri-sodium citrate, pH 5.6, VAPOR DIFFUSION, HANGING DROP, temperature 292K |
Crystallization Reagents
ID | crystal ID | solution ID | reagent name | concentration | details |
1 | 1 | 1 | PEG4000 | ||
2 | 1 | 1 | iso-propanol | ||
3 | 1 | 1 | tri-sodium citrate | ||
4 | 1 | 1 | H2O | ||
5 | 1 | 2 | PEG4000 | ||
6 | 1 | 2 | iso-propanol | ||
7 | 1 | 2 | tri-sodium citrate | ||
8 | 1 | 2 | H2O |