1YJP

Structure of GNNQQNY from yeast prion Sup35

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID13
Synchrotron site	ESRF
Beamline	ID13
Temperature [K]	100
Detector technology	CCD
Collection date	2004-06-12
Detector	MARRESEARCH
Wavelength(s)	0.975
Spacegroup name	P 1 21 1
Unit cell lengths	21.937, 4.866, 23.477
Unit cell angles	90.00, 107.08, 90.00

Refinement procedure

Resolution	22.440 - 1.800
R-factor	0.18139
Rwork	0.181
R-free	0.19014
Structure solution method	FOURIER SYNTHESIS
RMSD bond length	0.014
RMSD bond angle	1.228
Data reduction software	DENZO
Data scaling software	SCALEPACK
Refinement software	REFMAC

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	80.000	1.940
High resolution limit [Å]	1.800	1.800
Rmerge	0.204	0.491
Number of reflections	509
<I/σ(I)>	3.75	1.5
Completeness [%]	89.5	84.2
Redundancy	2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7	298	water, pH 7, VAPOR DIFFUSION, HANGING DROP, temperature 298K