1YFD
Crystal structure of the Y122H mutant of ribonucleotide reductase R2 protein from E. coli
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE BM1A |
Synchrotron site | ESRF |
Beamline | BM1A |
Temperature [K] | 100 |
Detector technology | IMAGE PLATE |
Collection date | 1997-07-27 |
Detector | MARRESEARCH |
Wavelength(s) | 0.873 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 74.040, 84.740, 115.030 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 19.460 - 1.900 |
R-factor | 0.17449 |
Rwork | 0.173 |
R-free | 0.20601 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1pfr |
RMSD bond length | 0.013 |
RMSD bond angle | 1.353 |
Data reduction software | XFIT |
Data scaling software | XDS |
Refinement software | REFMAC (5.2.0000) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 19.600 | 2.000 |
High resolution limit [Å] | 1.900 | 1.900 |
Number of reflections | 57178 | |
<I/σ(I)> | 10.8 | 6.8 |
Completeness [%] | 99.1 | 98.4 |
Redundancy | 3.3 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6 | 295 | PEG 4000, 0.1M MES, 0.4M NACL, 1mM EMTS, pH 6.0, VAPOR DIFFUSION, HANGING DROP, temperature 295K |