1YC6
Crystallographic Structure of the T=1 Particle of Brome Mosaic Virus
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ALS BEAMLINE 5.0.1 |
Synchrotron site | ALS |
Beamline | 5.0.1 |
Temperature [K] | 100 |
Detector technology | AREA DETECTOR |
Collection date | 2000-07-02 |
Detector | SDMS |
Wavelength(s) | 1.1 |
Spacegroup name | P 43 2 2 |
Unit cell lengths | 194.770, 194.770, 428.940 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 30.260 - 2.900 |
R-factor | 0.22 |
Rwork | 0.219 |
R-free | 0.23900 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1js9 |
RMSD bond length | 0.010 |
RMSD bond angle | 1.420 |
Data reduction software | SDMS |
Data scaling software | SCALEPACK |
Phasing software | X-PLOR |
Refinement software | CNS (1.1) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 30.300 | 3.000 |
High resolution limit [Å] | 2.900 | 2.900 |
Number of reflections | 181726 | |
<I/σ(I)> | 8.07 | 1.93 |
Completeness [%] | 99.5 | 96.5 |
Redundancy | 18.9 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 298 | sodium malonate, pH 7.5, VAPOR DIFFUSION, SITTING DROP, temperature 298K |