1YC1
Crystal Structures of human HSP90alpha complexed with dihydroxyphenylpyrazoles
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 5.0.3 |
| Synchrotron site | ALS |
| Beamline | 5.0.3 |
| Temperature [K] | 105 |
| Detector technology | IMAGE PLATE |
| Collection date | 2003-11-19 |
| Wavelength(s) | 1.0 |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 71.611, 89.000, 88.386 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 47.180 - 1.700 |
| R-factor | 0.19 |
| Rwork | 0.190 |
| R-free | 0.22700 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.044 |
| RMSD bond angle | 4.100 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | CNS (1.0) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 47.180 | 1.810 |
| High resolution limit [Å] | 1.700 | 1.700 |
| Rmerge | 0.036 | 0.253 |
| Number of reflections | 31248 | |
| <I/σ(I)> | 18.3 | |
| Completeness [%] | 99.4 | 98.9 |
| Redundancy | 4.7 | 4.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 9 | 277 | PEG 6000, LiCl, pH 9.0, VAPOR DIFFUSION, HANGING DROP, temperature 277K |
