1YAX

Cystal structure Analysis of S.typhimurium PhoQ sensor domain with Calcium

Experimental procedure

Experimental method	SAD
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 5.0.2
Synchrotron site	ALS
Beamline	5.0.2
Temperature [K]	93
Detector technology	CCD
Collection date	2003-11-18
Detector	ADSC QUANTUM 4
Wavelength(s)	0.9793
Spacegroup name	C 1 2 1
Unit cell lengths	118.078, 134.871, 58.444
Unit cell angles	90.00, 119.61, 90.00

Refinement procedure

Resolution	50.000 - 2.400
R-factor	0.244
Rwork	0.242
R-free	0.27952
Structure solution method	SAD and MR
RMSD bond length	0.013
RMSD bond angle	1.145
Data reduction software	HKL-2000
Data scaling software	SCALEPACK
Phasing software	SHARP
Refinement software	REFMAC (5.1.24)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.490
High resolution limit [Å]	2.400	2.400
Rmerge	0.040	0.253
Number of reflections	29394
<I/σ(I)>	35.29	3.68
Completeness [%]	95.0	72.2
Redundancy	3.6	2.6

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7	298	PEG 8000, tris-HCL, calcium acetate, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 298K
1	VAPOR DIFFUSION, HANGING DROP	7	298	PEG 8000, tris-HCL, calcium acetate, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 298K