1Y26
A-riboswitch-adenine complex
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X25 |
| Synchrotron site | NSLS |
| Beamline | X25 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2004-09-20 |
| Detector | MARRESEARCH |
| Wavelength(s) | 1.15 |
| Spacegroup name | P 21 21 2 |
| Unit cell lengths | 49.655, 155.036, 25.185 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 20.000 - 2.100 |
| R-factor | 0.23445 |
| Rwork | 0.231 |
| R-free | 0.29733 |
| Structure solution method | SIRAS |
| RMSD bond length | 0.016 |
| RMSD bond angle | 1.790 |
| Data reduction software | CrystalClear ((MSC/RIGAKU)) |
| Data scaling software | HKL-2000 |
| Phasing software | SHARP |
| Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 2.170 |
| High resolution limit [Å] | 2.100 | 2.100 |
| Rmerge | 0.069 | |
| Number of reflections | 11832 | |
| Completeness [%] | 97.8 | 0.934 |
| Redundancy | 10.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8 | 277 | Mg, PEG, pH 8, VAPOR DIFFUSION, HANGING DROP, temperature 277K |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8 | 277 | Mg, PEG, pH 8, VAPOR DIFFUSION, HANGING DROP, temperature 277K |
Crystallization Reagents
| ID | crystal ID | solution ID | reagent name | concentration | details |
| 1 | 1 | 1 | MgCl2, PEG | ||
| 2 | 1 | 2 | MgCl2, PEG |
