1Y23
Crystal structure of a member of HIT family of proteins from bacillus subtilis
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | NSLS BEAMLINE X4A |
Synchrotron site | NSLS |
Beamline | X4A |
Temperature [K] | 100 |
Detector technology | CCD |
Detector | ADSC QUANTUM 4 |
Wavelength(s) | 0.9800 |
Spacegroup name | P 31 2 1 |
Unit cell lengths | 86.444, 86.444, 180.046 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 19.960 - 2.300 |
R-factor | 0.2131 |
Rwork | 0.213 |
R-free | 0.24900 |
Structure solution method | SIRAS |
Starting model (for MR) | Experimental electron density map from Hg SIRAS density modified phases |
RMSD bond length | 0.007 |
RMSD bond angle | 1.300 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | MLPHARE |
Refinement software | CNS (1.1) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 19.960 | 2.380 |
High resolution limit [Å] | 2.300 | 2.300 |
Number of reflections | 35084 | |
Completeness [%] | 99.6 | 99.8 |
Redundancy | 17.5 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 5.5 | 291 | 19.5% Peg 4000, 0.5M Ammonium Acetate, 0.1M Sodium Citrate, pH 5.5, VAPOR DIFFUSION, HANGING DROP, temperature 291K |