1XZ2
wild-type hemoglobin deoxy no-salt
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU RU200 |
| Temperature [K] | 298 |
| Detector technology | AREA DETECTOR |
| Collection date | 1994-03-07 |
| Detector | SDMS |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 83.900, 112.100, 63.700 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 10.000 - 1.900 |
| R-factor | 0.17136 |
| Rwork | 0.168 |
| R-free | 0.20094 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1hbb |
| RMSD bond length | 0.014 |
| RMSD bond angle | 1.491 |
| Data reduction software | SDMS |
| Data scaling software | SDMS |
| Phasing software | X-PLOR |
| Refinement software | REFMAC (5.0) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.070 |
| High resolution limit [Å] | 1.930 | 1.930 |
| Rmerge | 0.059 | 0.185 |
| Number of reflections | 44690 | |
| <I/σ(I)> | 12.4 | 2.9 |
| Completeness [%] | 96.6 | 83.2 |
| Redundancy | 9 | 5.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | batch | 7 | 298 | 12% PEG 1450, 10 mg/ml Hb, pH 7.0, batch, temperature 298.0K |
