1XYS

CATALYTIC CORE OF XYLANASE A E246C MUTANT

Experimental procedure

Collection date	1993-12-27
Spacegroup name	P 43 21 2
Unit cell lengths	97.500, 97.500, 152.300
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	10.000 - 2.500
R-factor	0.2
RMSD bond length	0.017
RMSD bond angle	0.027
Data reduction software	DENZO
Refinement software	RESTRAIN

Data quality characteristics

	Overall
Low resolution limit [Å]	35.000
High resolution limit [Å]	2.500
Rmerge	0.078
Number of reflections	25370
Completeness [%]	97.7
Redundancy	5.3

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, hanging drop *	6.5 *	18 *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	20 (mg/ml)
2	1	reservoir	sodium cacodylate	0.1 (M)
3	1	reservoir	calcium acetate	200 (mM)
4	1	reservoir	2-mercaptoethanol	1 (mM)
5	1	reservoir	PEG8000	14-18 (%(w/v))