1XXT
The T-to-T High Transitions in Human Hemoglobin: wild-type deoxy Hb A (low salt, one test set)
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | RIGAKU RU200 |
Temperature [K] | 298 |
Detector technology | AREA DETECTOR |
Collection date | 1990-05-08 |
Detector | SDMS |
Spacegroup name | P 21 21 2 |
Unit cell lengths | 97.000, 99.300, 66.000 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 10.000 - 1.910 |
Rwork | 0.182 |
R-free | 0.23900 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1hbb |
RMSD bond length | 0.010 |
RMSD bond angle | 0.024 |
Data reduction software | SDMS |
Data scaling software | SDMS |
Phasing software | X-PLOR |
Refinement software | PROLSQ |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.050 |
High resolution limit [Å] | 1.910 | 1.910 |
Rmerge | 0.044 | 0.149 |
Number of reflections | 47999 | |
<I/σ(I)> | 13.7 | 3 |
Completeness [%] | 95.3 | 78.1 |
Redundancy | 6.7 | 2.8 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | 7 | 298 | 10% PEG 6000, 10 mM potassium phosphate, 100 mM potassium chloride, 3 mM sodium dithionite, 10 mg/ml hemoglobin, pH 7.0, BATCH, temperature 298K |