1XTY
Crystal structure of Sulfolobus solfataricus peptidyl-tRNA hydrolase
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE ID14-4 |
Synchrotron site | ESRF |
Beamline | ID14-4 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2003-09-15 |
Detector | ADSC QUANTUM 4 |
Wavelength(s) | 0.999 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 46.816, 67.232, 85.352 |
Unit cell angles | 90.00, 95.08, 90.00 |
Refinement procedure
Resolution | 46.600 - 1.800 |
R-factor | 0.205 |
Rwork | 0.205 |
R-free | 0.22400 |
Structure solution method | SIRAS |
RMSD bond length | 0.005 |
RMSD bond angle | 1.123 |
Data reduction software | MOSFLM |
Data scaling software | SCALA |
Phasing software | SOLVE |
Refinement software | CNS (1.0) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 1.850 |
High resolution limit [Å] | 1.800 | 1.800 |
Number of reflections | 48937 | |
Completeness [%] | 100.0 | 99.5 |
Redundancy | 5.4 | 3.8 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION | 2.8 | 297 | 0.8Ml SO4, 1.6% PEG 8000, pH 2.8, VAPOR DIFFUSION, temperature 297K |