1XPP
Crystal Structure of TA1416,DNA-directed RNA polymerase subunit L, from Thermoplasma acidophilum
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 19-ID |
Synchrotron site | APS |
Beamline | 19-ID |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2003-12-19 |
Detector | SBC-2 |
Wavelength(s) | 0.9793 |
Spacegroup name | P 1 2 1 |
Unit cell lengths | 59.700, 69.424, 66.293 |
Unit cell angles | 90.00, 116.77, 90.00 |
Refinement procedure
Resolution | 23.900 - 1.600 |
R-factor | 0.22355 |
Rwork | 0.219 |
R-free | 0.26351 |
Structure solution method | MAD |
RMSD bond length | 0.013 |
RMSD bond angle | 1.417 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | SOLVE |
Refinement software | REFMAC (5.2.0000) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 23.900 | 1.660 |
High resolution limit [Å] | 1.600 | 1.600 |
Rmerge | 0.069 | 0.347 |
Number of reflections | 60955 | |
<I/σ(I)> | 8.6 | 2.9 |
Completeness [%] | 95.2 | 91.7 |
Redundancy | 4.1 | 3.2 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 295 | potassium thiocyanate, PEG MME 2000, sodium cacodylate, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 295K |
1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 295 | potassium thiocyanate, PEG MME 2000, sodium cacodylate, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 295K |