1X7S
The X-ray crystallographic structure of the amyloidogenic variant TTR Tyr78Phe
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID14-1 |
| Synchrotron site | ESRF |
| Beamline | ID14-1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2003-06-20 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 0.934 |
| Spacegroup name | P 21 21 2 |
| Unit cell lengths | 42.719, 86.082, 63.799 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 10.000 - 1.550 |
| R-factor | 0.195 |
| Rwork | 0.194 |
| R-free | 0.23800 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1f86 |
| RMSD bond length | 0.009 |
| RMSD bond angle | 2.147 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | AMoRE |
| Refinement software | SHELXL-97 |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 51.110 | 1.630 |
| High resolution limit [Å] | 1.550 | 1.550 |
| Rmerge | 0.064 | 0.313 |
| Number of reflections | 33669 | |
| <I/σ(I)> | 5.7 | 2.4 |
| Completeness [%] | 98.0 | 96.7 |
| Redundancy | 8.8 | 6.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 4.8 | 287 | Ammonium Sulfate, Glycerol, Sodium Acetate, pH 4.8, VAPOR DIFFUSION, HANGING DROP, temperature 287K |
