1X07
Crystal structure of undecaprenyl pyrophosphate synthase in complex with Mg and IPP
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | RIGAKU MICROMAX-002+ |
Temperature [K] | 100 |
Detector technology | IMAGE PLATE |
Collection date | 2004-07-04 |
Detector | RIGAKU RAXIS IV |
Wavelength(s) | 1.5418 |
Spacegroup name | P 32 2 1 |
Unit cell lengths | 57.792, 57.792, 119.131 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 50.000 - 2.200 |
R-factor | 0.187 |
Rwork | 0.184 |
R-free | 0.25300 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | The monomer of A chain of PDB 1V7U |
RMSD bond length | 0.018 |
RMSD bond angle | 1.800 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | CNS |
Refinement software | CNS |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.280 |
High resolution limit [Å] | 2.200 | 2.200 |
Rmerge | 0.065 | 0.402 |
Number of reflections | 12171 | |
<I/σ(I)> | 24.4 | 3.3 |
Completeness [%] | 99.3 | 93.3 |
Redundancy | 4.6 | 3.3 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 298 | MgCl2, IPP, Tris-HCL, NaCl, Triton X-100, PEG35000, ethylene glycol, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 298K |