1WMK
Human death-associated kinase DRP-1, mutant S308D d40
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | EMBL/DESY, HAMBURG BEAMLINE X13 |
Synchrotron site | EMBL/DESY, HAMBURG |
Beamline | X13 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2003-10-15 |
Detector | MARRESEARCH |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 86.190, 143.290, 255.720 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 20.000 - 3.600 |
R-factor | 0.2773 |
Rwork | 0.276 |
R-free | 0.29577 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1SXO |
RMSD bond length | 0.006 |
RMSD bond angle | 0.874 |
Data reduction software | XDS |
Data scaling software | XDS |
Phasing software | MOLREP |
Refinement software | REFMAC (5.2.0000) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 20.000 | 3.700 |
High resolution limit [Å] | 3.600 | 3.600 |
Number of reflections | 37261 | |
<I/σ(I)> | 7.6 | 3.6 |
Completeness [%] | 99.2 | 99.9 |
Redundancy | 4.8 | 4.7 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 7.4 | 295 | PEG, sodium acetate, pH 7.4, VAPOR DIFFUSION, SITTING DROP, temperature 295K |