1WL8
Crystal structure of PH1346 protein from Pyrococcus horikoshii
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SPRING-8 BEAMLINE BL26B1 |
Synchrotron site | SPring-8 |
Beamline | BL26B1 |
Temperature [K] | 100 |
Detector technology | IMAGE PLATE |
Collection date | 2004-03-21 |
Detector | RIGAKU RAXIS |
Wavelength(s) | 1.0 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 46.808, 63.480, 65.161 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 28.990 - 1.450 |
R-factor | 0.15638 |
Rwork | 0.155 |
R-free | 0.17365 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1qdl |
RMSD bond length | 0.011 |
RMSD bond angle | 1.481 |
Data reduction software | HKL-2000 |
Data scaling software | SCALEPACK |
Phasing software | AMoRE |
Refinement software | REFMAC (5.1.27) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 40.000 | 1.500 |
High resolution limit [Å] | 1.450 | 1.450 |
Rmerge | 0.070 | 0.250 |
Number of reflections | 33220 | |
<I/σ(I)> | 14.7 | |
Completeness [%] | 99.9 | 100 |
Redundancy | 7 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | MICROBATCH | 4.8 | 295 | Acetate, PEG, pH 4.8, microbatch, temperature 295.0K |