1WIQ
STRUCTURE OF T-CELL SURFACE GLYCOPROTEIN CD4, TRIGONAL CRYSTAL FORM
Experimental procedure
Source type | SYNCHROTRON |
Source details | CHESS BEAMLINE F1 |
Synchrotron site | CHESS |
Beamline | F1 |
Temperature [K] | 298 |
Spacegroup name | P 31 2 1 |
Unit cell lengths | 126.000, 126.000, 205.000 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 8.000 - 5.000 |
R-factor | 0.422 |
Rwork | 0.422 |
R-free | 0.44200 |
Structure solution method | MOLECULAR REPLACEMENT PLUS ANOMALOUS DIFFRACTION. |
RMSD bond length | 0.015 |
RMSD bond angle | 2.300 |
Data reduction software | DENZO |
Data scaling software | CCP4 |
Phasing software | X-PLOR |
Refinement software | X-PLOR |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 8.000 * | |
High resolution limit [Å] | 5.000 * | |
Rmerge | 0.086 * | 0.295 * |
Number of reflections | 4289 * | |
Completeness [%] | 67.3 * | 49.9 * |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | Vapor diffusion, hanging drop * | 8.2 * | 20 * | Kwong, P.D., (1990) Proc. Natl. Acad. Sci. U.S.A., 87, 6423. * |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | drop | PEG400 | 0.23 (%) | |
2 | 1 | drop | ammonium phosphate | 183 (mM) | |
3 | 1 | drop | sodium phosphate | 40 (mM) | |
4 | 1 | drop | Tris-HCl | 10 (mM) | |
5 | 1 | drop | 3.1 (mM) | ||
6 | 1 | drop | sCD4 | 4.0 (mg/ml) | |
7 | 1 | reservoir | ammonium sulfate | 39.0 (%sat) | |
8 | 1 | reservoir | Tris-HCl | 100 (mM) | |
9 | 1 | reservoir | 74 (mM) |