1WIP
STRUCTURE OF T-CELL SURFACE GLYCOPROTEIN CD4, MONOCLINIC CRYSTAL FORM
Experimental procedure
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X4A |
| Synchrotron site | NSLS |
| Beamline | X4A |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Detector | FUJI |
| Spacegroup name | P 1 2 1 |
| Unit cell lengths | 100.500, 123.400, 100.600 |
| Unit cell angles | 90.00, 103.40, 90.00 |
Refinement procedure
| Resolution | 8.000 - 4.000 |
| R-factor | 0.452 |
| Rwork | 0.452 |
| R-free | 0.42700 |
| Structure solution method | MOLECULAR REPLACEMENT PLUS ANOMALOUS DIFFRACTION. |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | REPLACE |
| Refinement software | X-PLOR |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 8.000 * | |
| High resolution limit [Å] | 4.000 * | |
| Rmerge | 0.062 * | 0.254 * |
| Number of reflections | 13474 * | |
| Completeness [%] | 76.0 * | 45.8 * |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion, hanging drop * | 7.5 * | 4 * | Kwong, P.D., (1990) Proc. Natl. Acad. Sci. U.S.A., 87, 6423. * |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | PEG400 | 2.0 (%) | |
| 2 | 1 | drop | ammonium sulfate | 15 (mM) | |
| 3 | 1 | drop | sodium cacodylate | 6.0 (mM) | |
| 4 | 1 | drop | Tris-HCl | 5.0 (mM) | |
| 5 | 1 | drop | sCD4 | 3.1 (mg/ml) | |
| 6 | 1 | reservoir | PEG400 | 30.0 (%) | |
| 7 | 1 | reservoir | Tris-HCl | 100 (mM) | |
| 8 | 1 | reservoir | 29.6 (mM) |
